On tissues
Monitor Ligand – tissue interactions in real-time
Perform real-time binding studies (e.g., affinity determination, immunohistochemistry (IHC), uptake/retention) with LigandTracer by adding an animal organ or fresh/frozen tissue sections directly to the Petri Dish.
Whether you are doing 𝙚𝙭 𝙫𝙞𝙫𝙤 studies on xenografts, human tumour, or organ tissues (like brain, heart, muscle), LigandTracer brings your research one step closer to 𝙞𝙣 𝙫𝙞𝙫𝙤 conditions.
The time-resolved analysis of tissue binders provides new insight into their interaction properties and helps to optimize IHC protocols, including ideal incubation times and concentrations.
Key applications
- Estimate kinetics and affinity
- Investigate specificity of tissue binders
- Optimize manual IHC protocols
- Follow metabolism in real time
- Study interaction heterogeneity
Key publications
These publications illustrate the application well. Click here for more examples.
Evaluation of real-time immunohistochemistry and Interaction Map as an alternative objective assessment of HER2 expression in human breast cancer tissue.
Gedda L, Björkelund H, Lebel L, Asplund A, Dubois L, Wester K, Penagos N, Malmqvist M, Andersson K.
Appl Immunohistochem Mol Morphol. 2013. 21(6):497-505.
Summary
PubMed
Effect of starvation on brain glucose metabolism and 18F-2-fluoro-2- deoxyglucose uptake.
Buschiazzo A, Cossu V, Bauckneht M, Orengo A, Piccioli P, Emionite L, Bianchi G, Grillo F, Rocchi A, Di Giulio F, Fiz F, Raffaghello L, Nobili F, Bruno S, Caviglia G, Ravera S, Benfenati F, Piana M, Morbelli S, Sambuceti G, Marini C.
Eur J Nucl Med Mol Imaging. 2019. 46(5):1184-1196.
Summary
PubMed
Bioinspired Bottlebrush Polymers Effectively Alleviate Frictional Damage Both In Vitro and In Vivo.
Pham D A, Wang C S, Séguy L, Zhang H, Benbabaali S, Faivre J, Sim S, Xie G, Olszewski M, Rabanel J M, Moldovan F, Matyjaszewski K, Banquy X.
Adv. Mater. 2024 June;36:2401689.
Summary
PubMed
Examples of targets
